H7789
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 | Immunoblotting Acid-extracted fraction of HL60 cells were separated on SDS-PAGE and blotted with Anti-Acetyl Histone H1.4 [Ac-Lys26] (Cat. No. H7789). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:2,000 Lanes 1-3: HL60 cells 2: Inhibition with acetyl-Histone H1.4 [Ac-Lys26] (human 22-33) peptide (2 μg/mL) 3: Inhibition with Histone H1.4 (human 22-33) peptide (2 μg/mL) |
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H7789
Sigma
Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-H1 Histone family, member 4, Anti-H1e, Anti-Histone 1, Anti-Histone H1b |
Description
| Immunogen | synthetic acetylated peptide corresponding to amino acids 22-33 (Ac-Lys26) of human histone H1.4. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~35 kDa |
| species reactivity | human |
| application(s) | immunoblotting: 0.5-1 μg/mL using acid-extracted fraction of HL60 cells |
| Gene Information | human ... HIST1H1E(3008) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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